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Coat protein-RNA interaction in vivo and the biotechnological use of VLPs
Kratochvílová, Kateřina ; Moravec, Tomáš (advisor) ; Hála, Michal (referee)
The Tobacco mosaic virus (TMV) is a simple and frequently used model virus which has been studied already more over than 130 years. Due to the intensive study of this virus the details of its infectious cycle, genomic information and also the structure of the created viral particle as well as the mechanism of its creation are known today. The process of encapsidation (viral particle formation) is sufficiently described in the in vitro conditions. In the in vitro conditions the origin of assembly (OAS) was also described. The OAS was identified in the coding sequence of the gene for the movement protein (MP). The importance of replication centers (replication factories) has also been supposed. The aim of the diploma thesis was to study the specificity of the interaction of RNA and coat protein in the process of the particle assembly taking place directly inside the plants. The experiments were performed to verify the necessity of presence of OAS sequence in process of initiation of viral encapsidation. The effect of the cell compartmentation on this process has also been studied. Based on several viral systems (the Tobacco mosaic virus, the Potato virus X, the Bean yellow dwarf virus and Cowpea mosaic virus) gene constructs were created. These constructs enables to study this idea at the molecular...
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The use virus derived vectors for the expression of proteins in plants
Kratochvílová, Kateřina ; Moravec, Tomáš (advisor) ; Holá, Dana (referee)
The tobacco mosaic virus (TMV) is one of the most studied virus. Its properties (simplicity, self assembly of viral capsid, the ability to proliferate in a large variety of hosts) offers a lot of applications in various scientific or biological fields. Gene vectors based on the tobacco mosaic virus represent a very good way for expressing proteins in plants. Because the tobacco mosaic virus is well researched and a large number of his properties have already been discovered, it is used for nanotechnologic sectors in this period. Not only for the formation of nanoparticles, but also for example for a potential production of vaccines in plants. In this paper I summarize the known information about the process of self assembly of tobacco mosaic virus and the possibility of using this virus in the emerging fields of biology. Key words Tobacco mosaic virus, self assembly, viral vector, nanotechnology
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Viral vectors based on tobacco mosaic virus for the expression of recombinant proteins in plants.
Kroupa, Daniel ; Moravec, Tomáš (advisor) ; Plchová, Helena (referee)
(eng): Over the past few decades, plant viruses and vectors derived from them have become not only an integral part of the work in laboratories, but also began to be used commercially for the production of vaccines, drugs, enzymes and other proteins / peptides. One of the most commonly used viruses for the expression of heterologous proteins is tobacco mosaic virus. In this work, I would like to focus on plant viruses and vectors derived therefrom. Compare it with other expression systems used in plants and describe how their benefits could be further used and combined with modern methods of cloning. Key words: viral vector, expression, Tobacco mosaic virus, Cowpea mosaic virus, Golden gate cloning
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